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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial position in selling cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two added splice variants were described but in-depth nucleotide sequence details isn't nonetheless offered.

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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.

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This information website outlines even more attainable optimization of cellular and immunoassay parameters to ensure the absolute best final results are...

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Antibodies sure to the blocking peptide now not bind into the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.

Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities to be a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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